Reporter

Part:BBa_K2084006:Design

Designed by: Maya Lemmon-Kishi   Group: iGEM16_Pittsburgh   (2016-10-16)


pT3 Controlled GFP


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 694


Design Notes

We had to create primers to PCR amplify the sequence out so that it was in the BioBrick compatible backbone.


Source

T3 RNAP promoter is from T3 Bacteriophage GFP is from... We obtained the whole sequence from AddGene and Christopher Voigt.

References